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10

Aug
2024

By sakiko

DNA Purification

On 10, Aug 2024 | No Comments | In Website | By sakiko

DNA purification is a step in a process of sample preparation that removes enzymes, salts and other contaminants from lysed samples or PCR products before subsequent applications such as cloning or sequencing. It also eliminates unwanted PCR artifacts, like primer dimers and nucleotides not integrated. DNA purification is a critical step in molecular biology that requires https://mpsciences.com/2021/04/23/dna-purification-processes-for-different-applications/ careful planning to produce top-quality, reliable results.

There are several different approaches to cleaning DNA. Traditional DNA isolation methods require many steps including leukocyte isolation or red blood cell lysis in order to remove heme proteins that block the PCR reaction, deproteinization and RNAse treatment, ethanol and isopropanol precipitation, and then DNA elimination. These methods require specialized equipment, for example, an electrophoresis system and biosafety cabinets, due to the intercalating dyes used in gel electrophoresis.

Other methods of DNA purification use spin columns or plates with 96 wells that separate particles that are contaminated by adhering to the surface. These methods can be lengthy especially when you have a large number of samples or if the columns need to be manually refilled.

Dipsticks reduce the number of sample processing steps from six to three. They bind nucleic acids with the cellulose-based waxy material and subsequently release them in the presence of water. This method is particularly effective in low-resource settings, such as remote locations and teaching labs. Its simplicity and speed (30 s for each sample) is a great fit for molecular diagnostics like diagnostics, genotype screening, and heterozygosity testing.

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